白枕鹤精液冷冻技术的研究

白枕鹤（ＧＲＵＳＶ　ＩＰＩＯ）羽毛青灰，红面，体型健美优雅，具有很高的观赏价值，已被国家列为二级保护动物。对白枕鹤类的人工授精、孵化、育雏以及冷冻精液技术的研究，可促进人工饲养条件下鹤类的增殖和种群的繁衍。本研究采用Ｂｅｌｔｓｖｉｌｌｅ稀释液做为基础液，４—６％ＤＭＳＯ做为冷冻保护剂，按常规冷冻法冻精配种受胎率达４０％，此项研究填补了我国在鹤类精液冷冻技术方面的空白。     

Comparative infectivity for axenic and specific-pathogen-free chickens of O2 Escherichia coli strains with or without virulence factors

Adhesion to epithelial respiratory cells, iron acquisition, and production of K1 polysaccharide capsules have been proposed as potential virulence factors of avian Escherichia coli. These factors were studied by inoculating groups of axenic or specific-pathogen-free (SPF) chickens intratracheally with O2 E. coli strains after previous challenge with a wild strain of infectious bronchitis virus (IBV). In all experiments, the association between IBV and an E. coli strain endowed with the three virulence factors previously mentioned resulted in the most severe pathological effects, as measured by mortality, weight gains, lesions, and reisolation of E. coli from internal organs. An E. coli strain devoid of virulence factors was able only to induce mild pathological effects restricted to the respiratory tract when combined with IBV. Both E. coli strains were more invasive in axenic chickens than in SPF chickens. These results confirm the probable involvement of the three factors studied in the pathogenic properties of avian E. coli. This model can be used to assess the role of virulence factors, by comparing pairs of positive and negative isogenic strains.     

Soil mixing in the East Anglian fens

Abstract. Experiments were started in 1967 to investigate the effects of soil mixing on fen peat soil. Peaty topsoil was mixed with mineral subsoil to a maximum depth of 80 cm at two sites, one with a clay subsoil, the other sand. Mixing was done on a commercial scale with a range of implements. After initial increases, yields of arable crops on both mixed soils were subsequently similar to those on unmixed soil. Residual herbicide activity was enhanced on mixed soils, and evidence of peat conservation was obtained at one site. The practice has not been taken up commercially because of the high capital cost of mixing and lack of serious problems which had been predicted following peat wastage.     

Situation of glasshouse pests in Hungary1

The area of protected crops in Hungary is about 5300 ha. Most of it is under plastic, the area under glass being only about 250 ha. Vegetable growing makes up 80%, mostly on private farms. The main crops are capsicum, tomato and cucumber. On relatively small areas, ornamentals, principally cut flowers, are produced: carnation, gerbera, rose and chrysanthemum. The three most important, commonly occurring pests are Trialeurodes vaporariorum, Tetranychus urticae and aphids (Aphis gossypii, Myzus persicae, A. nasturtii). Polyphagotarsonemus latus, Liriomyza trifolii and Aculops lycopersici caused considerable economic damage during the eighties. However, in the last few years these pests have not been observed in any crops. Two new glasshouse pests have been described from ornamentals during the last 2 years in Hungary: Frankliniella occidentalis and Bemisia tabaci. Their accidental appearance in vegetable crops could lead to direct economic loss, besides endangering biological control systems.     

Parosteal osteosarcoma of the mandible in a dog

A mandibular parosteal osteosarcoma was diagnosed in a 7-year-old Collie with prolapse of the third eyelid. Diagnosis was made by histologic evaluation of a mass removed by surgical excision. The dog was euthanatized because of local recurrence of the tumor, with metastasis to the lungs. Parosteal osteosarcomas are rarely reported in dogs and usually involve long bones in human beings.     

A simple method for the quantitative determination of sperm aggregation

Described in this paper are 2 methodological variants for photometric recording of sperm aggregation. Heparin was used to induce aggregation. One of the working principles was related to aggregation-associated alteration of turbidity in an agitated cell suspension, measured by the KZM-1 coagulation-time meter, in analogy to measurement of thrombocyte aggregation. The 2nd variant was based on variation of cloudiness due to sedimentation of aggregates in a non-agitated suspension. Both methods provided equally valid information. They characterised both intensity of aggregation as a combined effect of the number and size of aggregates as well as the rate of aggregation. Photometric recording has proved to be an objectivated method for quantitative assessment of aggregation. Its use is proposed for studies into capacitation or immunological response of spermatozoa.     

Evaluation of the testosterone response to hCG and the identification of a presumed anorchid dog

A two-year-old dog was presented with no scrotal testes. There was no history of castration but at laparotomy no testes were found. To confirm that the dog was truly anorchid a human chorionic gonadotrophin stimulation test was performed. This was then validated in normal entire and castrated dogs.